Abstract Background The relative ease of targeted gene disruption in the social amoeba Dictyostelium has stimulated its widespread use as an experimental organism for cell and developmental biology. However. the field has been hamstrung by the lack of techniques to recombine disrupted genes. Results We describe new techniques for parasexual fusion of strains in liquid medium. https://allfixelectricales.shop/product-category/audio-turntable-stylus/